Category Archives : Papers


“Degenerate adaptor sequences for detecting PCR duplicates in reduced representation sequencing data improve genotype-calling accuracy” paper published!

Together with our collaborators from the National University of Singapore we just published a paper describing an improved method for making double-digest RAD-tag libraries. By adding a small run of degenerate nucleotides to the end of one of the Illumina indexes, you can barcode each individual ligation reaction and determine how many molecules participate in [...]

2014-08-28 07-52-07 Degenerate adaptor sequences for detecting PCR duplicates in reduced representation sequencing data imp

Extracted carpenter ant

It belongs in a museum!

Over lunch during one of his visits to OIST, my former boss Sydney Brenner suggested that I try a ribo-tailing technique for attaching adaptors to short DNA molecules. The idea is that the action of terminal transferase on riboguanidines creates a 3′ tail of controlled length, which can be used for attaching adaptors and synthesizing [...]


Host-associated genomic differentiation paper recommended by F1000

It was a long, hard road, but our paper is getting some recognition! F1000′s Dr. Ian Wang writes that “this finding highlights the importance of considering multiple factors, including dispersal, population sizes, spatial variation in ecologically-important traits, and population histories, for understanding how ecological adaptation contributes to patterns of differentiation throughout the genome.” I hope [...]