Daily Archives: August 27, 2014


“Degenerate adaptor sequences for detecting PCR duplicates in reduced representation sequencing data improve genotype-calling accuracy” paper published!

Together with our collaborators from the National University of Singapore we just published a paper describing an improved method for making double-digest RAD-tag libraries. By adding a small run of degenerate nucleotides to the end of one of the Illumina indexes, you can barcode each individual ligation reaction and determine how many molecules participate in [...]

2014-08-28 07-52-07 Degenerate adaptor sequences for detecting PCR duplicates in reduced representation sequencing data imp