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Eco Evo Lab

Better living through sequencing chemistry

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Methods

Overview

Please let if you find any errors, or have suggestions on how we can improve the protocols below.

Laboratory protocols

DNA extraction

      Easily automatable procedure for DNA extraction.

      Useful for large-insert libraries for de novo genome sequencing.


Sequencing library preparation

      This protocol can be used for degraded DNA

      Citation: Tin et al. PLoS ONE 2014, 9(5): e96793

      Related to the EcoRI RAD-tag protocol above, but used for whole genome shotgun sequencing.

      Citation: Tin et al. PLoS ONE 2014, 9(5): e96793

      Modification of original RAD-tag protocol to reduce error and bias due to PCR amplification.       Citation: Mikheyev et al. Nature Communications, 6:7991

      Citation: Aird et al. BMC Genomics 2013, 14:790

       High-throughput protocol related to the EcoRI RAD-tag procedure above. Optimized for processing 96 samples at one using a liquid handling robot.

      Citation: Tin et al. Molecular Ecology Resources 2015, 14:790


General protocols

Useful for processing large numbers of samples at once.

Most accurate measure of overall dsDNA concentration.